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KMID : 1132720120100030206
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Genomics & Informatics
2012 Volume.10 No. 3 p.206 ~ p.211
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DNA Barcoding of Fish, Insects, and Shellfish in Korea
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Kim Dae-Won
Yoo Won-Gi
Park Hyun-Chul
Yoo Hye-Sook
Kang Dong-Won
Jin Seon-Deok
Min Hong-Ki
Paek Woon-Kee
Lim Jeong-Heui
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Abstract
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DNA barcoding has been widely used in species identification and biodiversity research. A short fragment of the mitochondrial cytochrome c oxidase subunit I (COI) sequence serves as a DNA bio-barcode. We collected DNA barcodes, based on COI sequences from 156 species (529 sequences) of fish, insects, and shellfish. We present results on phylogenetic relationships to assess biodiversity the in the Korean peninsula. Average GC% contents of the 68 fish species (46.9%), the 59 shellfish species (38.0%), and the 29 insect species (33.2%) are reported. Using the Kimura 2 parameter in all possible pairwise comparisons, the average interspecific distances were compared with the average intraspecific distances in fish (3.22 vs. 0.41), insects (2.06 vs. 0.25), and shellfish (3.58 vs. 0.14). Our results confirm that distance-based DNA barcoding provides sufficient information to identify and delineate fish, insect, and shellfish species by means of all possible pairwise comparisons. These results also confirm that the development of an effective molecular barcode identification system is possible. All DNA barcode sequences collected from our study will be useful for the interpretation of species-level identification and community-level patterns in fish, insects, and shellfish in Korea, although at the species level, the rate of correct identification in a diversified environment might be low.
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KEYWORD
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cytochrome c oxidase subunit I, mitochondrial DNA, molecular taxonomy, taxonomic DNA barcoding
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